Difference between revisions of "Family-based Association Exercise"
From Statistical Genetics Courses
Serveradmin (Talk | contribs) (Created page with "__NOTITLE__ ==Family-based Association Exercise== cd exercises/cordell/FASTLMM_GCTA ls -l") |
Serveradmin (Talk | contribs) |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
==Family-based Association Exercise== | ==Family-based Association Exercise== | ||
| − | + | cd exercises/cordell/FASTLMM_GCTA | |
| − | cd exercises/cordell/FASTLMM_GCTA | + | ls -l |
| − | ls -l | + | plink --bfile quantfamdata --assoc --out plinkresults |
| + | R | ||
| + | res1<-read.table("plinkresults.qassoc", header=T) | ||
| + | head(res1) | ||
| + | source("qqmanHJCupdated.R") | ||
| + | jpeg("mh1.jpeg", height=500, width=800) | ||
| + | manhattan(res1, pch=20, suggestiveline=F, genomewideline=F, ymin=2, cex.x.axis=0.65, colors=c("black","dodgerblue"), cex=0.5) | ||
| + | dev.off() | ||
| + | jpeg("qqplot1.jpeg", height=500, width=500) | ||
| + | qq(res1$P) | ||
| + | dev.off() | ||
| + | chi<-(qchisq(1-res1$P,1)) | ||
| + | lambda=median(chi)/0.456 | ||
| + | lambda | ||
| + | fastlmmc -bfile quantfamdata -pheno quantfamdata.fam -mpheno 4 -bfileSim quantfamdata -ML -out FLMMresults | ||
| + | R | ||
| + | res2<-read.table("FLMMresults", header=T) | ||
| + | head(res2) | ||
| + | chi<-(qchisq(1-res2$Pvalue,1)) | ||
| + | lambda=median(chi)/0.456 | ||
| + | lambda | ||
| + | new<-data.frame(res2$SNP, res2$Chromosome, res2$Position, res2$Pvalue) | ||
| + | names(new)<-c("SNP", "CHR", "BP", "P") | ||
| + | head(new) | ||
| + | jpeg("qqplot2.jpeg", height=500, width=500) | ||
| + | qq(new$P) | ||
| + | dev.off() | ||
| + | jpeg("mh2.jpeg", height=500, width=800) | ||
| + | manhattan(new, pch=20, suggestiveline=F, genomewideline=F, ymin=2, cex.x.axis=0.65, colors=c("black","dodgerblue"), cex=0.5) | ||
| + | dev.off() | ||
Latest revision as of 19:33, 24 January 2019
Family-based Association Exercise
cd exercises/cordell/FASTLMM_GCTA
ls -l
plink --bfile quantfamdata --assoc --out plinkresults
R
res1<-read.table("plinkresults.qassoc", header=T)
head(res1)
source("qqmanHJCupdated.R")
jpeg("mh1.jpeg", height=500, width=800)
manhattan(res1, pch=20, suggestiveline=F, genomewideline=F, ymin=2, cex.x.axis=0.65, colors=c("black","dodgerblue"), cex=0.5)
dev.off()
jpeg("qqplot1.jpeg", height=500, width=500)
qq(res1$P)
dev.off()
chi<-(qchisq(1-res1$P,1))
lambda=median(chi)/0.456
lambda
fastlmmc -bfile quantfamdata -pheno quantfamdata.fam -mpheno 4 -bfileSim quantfamdata -ML -out FLMMresults
R
res2<-read.table("FLMMresults", header=T)
head(res2)
chi<-(qchisq(1-res2$Pvalue,1))
lambda=median(chi)/0.456
lambda
new<-data.frame(res2$SNP, res2$Chromosome, res2$Position, res2$Pvalue)
names(new)<-c("SNP", "CHR", "BP", "P")
head(new)
jpeg("qqplot2.jpeg", height=500, width=500)
qq(new$P)
dev.off()
jpeg("mh2.jpeg", height=500, width=800)
manhattan(new, pch=20, suggestiveline=F, genomewideline=F, ymin=2, cex.x.axis=0.65, colors=c("black","dodgerblue"), cex=0.5)
dev.off()
