Difference between revisions of "AdvGeneMap2018Commands"

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(→‎Plink Part 1 - Data QC)
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__NOTITLE__
 
__NOTITLE__
  
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==Plink Part 1 - Data QC==
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==Plink - Part 1 - Data QC==
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  #PLINK
 
  #PLINK
 
  plink --file GWAS
 
  plink --file GWAS
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  plink --file GWAS_clean3 --het
 
  plink --file GWAS_clean3 --het
 
  ###### in R
 
  ###### in R
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  Dataset <- read.table("plink.het", header=TRUE, sep="", na.strings="NA", dec=".", strip.white=TRUE)
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  Dataset
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mean(Dataset$F)
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sd(Dataset$F)
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==Plink - Part 2 - Controlling for Substructure==
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jpeg("hist.jpeg", height=1000, width=1000)
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hist(scale(Dataset$F), xlim=c(-4,4))
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dev.off()
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q()
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######
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plink --file GWAS_clean3 --pheno pheno.txt --pheno-name Aff --hardy
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##### in R
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hardy = read.table("plink.hwe", header = T)
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names(hardy)
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hwe_prob = hardy[which(hardy$P < 0.0000009),]
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hwe_prob
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q()
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##########
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plink --file GWAS_clean3 --exclude HWE_out.txt --recode --out GWAS_clean4
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===Plink Part 2 - Controlling for Substructure===
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plink --file GWAS_clean4 --genome --cluster --mds-plot 10
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  plink --file GWAS_clean4 --genome --cluster --mds-plot 10
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   #### in R
 
   #### in R
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   mydata = read.table("mds_components.txt", header=T)
 
   mydata = read.table("mds_components.txt", header=T)
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   mydata$pch[mydata$Group==1 ] observed¶   lobs
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   mydata$pch[mydata$Group==1 ] <-15
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expected lexp plot(c(0,7), c(0,7), col="red", lwd=3, type="l", xlab="Expected (-logP)", ylab="Observed (-logP)", xlim=c(0,max(lobs)), ylim=c(0,max(lobs)), las=1, xaxs="i", yaxs="i", bty="l", main = title)
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  mydata$pch[mydata$Group==2 ] <-16
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points(lexp, lobs, pch=23, cex=.4, bg="black") }
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  mydata$pch[mydata$Group==3 ] <-2
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  jpeg("mds.jpeg", height=500, width=500)
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  plot(mydata$C1, mydata$C2 ,pch=mydata$pch)
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  dev.off()
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  q()
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  ######
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  plink --file GWAS_clean4 --pheno pheno.txt --pheno-name Aff --logistic --adjust --out unadj
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  plink --file GWAS_clean4 --genome --cluster --pca 10 header
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  plink --file GWAS_clean4 --pheno pheno.txt --pheno-name Aff --covar plink.eigenvec --covar-name PC1 --logistic --adjust --out PC1
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  plink --file GWAS_clean4 --pheno pheno.txt --pheno-name Aff --covar plink.eigenvec --covar-name PC1-PC2 --logistic --adjust --out PC1-PC2
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  #### in R
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  broadqq <-function(pvals, title)
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  {
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  observed <- sort(pvals)
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   lobs <- -(log10(observed))
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  expected <- c(1:length(observed))
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  lexp <- -(log10(expected / (length(expected)+1)))
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  plot(c(0,7), c(0,7), col="red", lwd=3, type="l", xlab="Expected (-logP)", ylab="Observed (-logP)", xlim=c(0,max(lobs)), ylim=c(0,max(lobs)), las=1, xaxs="i", yaxs="i", bty="l", main = title)
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  points(lexp, lobs, pch=23, cex=.4, bg="black") }
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   jpeg("qqplot_compare.jpeg", height=1000, width=500)
 
   jpeg("qqplot_compare.jpeg", height=1000, width=500)
 
   par(mfrow=c(2,1))
 
   par(mfrow=c(2,1))
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   broadqq(aff_C1C2.add.p, "Some Trait Adjusted for PC1 and PC2")
 
   broadqq(aff_C1C2.add.p, "Some Trait Adjusted for PC1 and PC2")
 
   dev.off()
 
   dev.off()
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   gws_unadj = aff_unadj[which(aff_unadj$P < 0.0000001),]
 
   gws_unadj = aff_unadj[which(aff_unadj$P < 0.0000001),]
 
   gws_unadj
 
   gws_unadj
 
   gws_adjusted = aff_C1C2[which(aff_C1C2$P < 0.0000001),]
 
   gws_adjusted = aff_C1C2[which(aff_C1C2$P < 0.0000001),]
 
   gws_adjusted
 
   gws_adjusted

Revision as of 14:50, 23 January 2018

Plink - Part 1 - Data QC

#PLINK
plink --file GWAS
plink --file GWAS --mind 0.10 --recode --out GWAS_clean_mind
plink --file GWAS_clean_mind --maf 0.05 --recode --out MAF_greater_5
plink --file GWAS_clean_mind --exclude MAF_greater_5.map --recode --out MAF_less_5
plink --file MAF_greater_5 --geno 0.05 --recode --out MAF_greater_5_clean
plink --file MAF_less_5 --geno 0.01 --recode --out MAF_less_5_clean
plink --file MAF_greater_5_clean --merge MAF_less_5_clean.ped MAF_less_5_clean.map --recode --out GWAS_MAF_clean
plink --file GWAS_MAF_clean --mind 0.03 --recode --out GWAS_clean2
plink --file GWAS_clean2 --check-sex --out GWAS_sex_checking
#### in R - open R by simply typing R
setwd("to_your_working_directory/")
sexcheck = read.table("GWAS_sex_checking.sexcheck", header=T)
names(sexcheck)
sex_problem = sexcheck[which(sexcheck$STATUS=="PROBLEM"),]
sex_problem
q()
##################################
plink --file GWAS_clean2 --genome --out duplicates
#### in R
setwd("to_your_working_directory/")
dups = read.table("duplicates.genome", header = T)
problem_pairs = dups[which(dups$PI_HAT > 0.4),]
problem_pairs
problem_pairs = dups[which(dups$PI_HAT > 0.05),]
myvars = c("FID1", "IID1", "FID2", "IID2", "PI_HAT")
problem_pairs[myvars]
q()
######
plink --file GWAS_clean2 --remove IBS_excluded.txt --recode --out GWAS_clean3
plink --file GWAS_clean3 --het
###### in R
Dataset

Plink - Part 2 - Controlling for Substructure

plink --file GWAS_clean4 --genome --cluster --mds-plot 10
 #### in R
 mydata = read.table("mds_components.txt", header=T)
 mydata$pch[mydata$Group==1 ]  	observed¶   	lobs

expected ¶ lexp plot(c(0,7), c(0,7), col="red", lwd=3, type="l", xlab="Expected (-logP)", ylab="Observed (-logP)", xlim=c(0,max(lobs)), ylim=c(0,max(lobs)), las=1, xaxs="i", yaxs="i", bty="l", main = title) points(lexp, lobs, pch=23, cex=.4, bg="black") } 


 jpeg("qqplot_compare.jpeg", height=1000, width=500)
 par(mfrow=c(2,1))
 aff_unadj<-read.table("unadj.assoc.logistic", header=TRUE)
 aff_unadj.add.p<-aff_unadj[aff_unadj$TEST==c("ADD"),]$P
 broadqq(aff_unadj.add.p,"Some Trait Unadjusted")
 aff_C1C2<-read.table("PC1-PC2.assoc.logistic", header=TRUE)
 aff_C1C2.add.p<-aff_C1C2[aff_C1C2$TEST==c("ADD"),]$P
 broadqq(aff_C1C2.add.p, "Some Trait Adjusted for PC1 and PC2")
 dev.off()
 gws_unadj = aff_unadj[which(aff_unadj$P < 0.0000001),]
 gws_unadj
 gws_adjusted = aff_C1C2[which(aff_C1C2$P < 0.0000001),]
 gws_adjusted
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